Examination of Photo-, Mixo-, and Heterotrophic Cultivation Conditions on Haematococcus pluvialis Cyst Cell Germination

The microalgae Haematococcus pluvialis is used for the biotechnological production of astaxanthin. red carotenoid accumulates in cytoplasm under unfavorable conditions. Astaxanthin synthesis associated with transformation motile vegetative cells into non-motile cyst cells. In industrial process, after harvesting, are mechanically disrupted, dried, and finally, astaxanthin extracted supercritical CO2. germination represents an interesting alternative, replacing mechanical cell wall disruption. When exposed to favorable growth conditions, occurs zoospores released a certain time. These show much weaker matrix compared this study, phototrophic, mixotrophic, heterotrophic conditions was examined. Glucose as carbon source mixotrophic germination. Applying up 80% were zoospore stage 49 h start germination, extraction yields 50% achieved using solvent ethyl acetate from algal broth containing zoospores. An yield 64% could be by doubling nitrate concentration combining cultivation.